Technology Core

LiPiCS services develops comprehensive approaches to study protein-protein interactions based on Bimolecular Fluorescence Complementation method (BiFC*). This technology uses the ability of fluorescent protein to be cleave in two non-fluorescent part. Upon spatial proximity those two parts will complement to reform a functional fluorescent signal. 

B-LiPiCS

LiPiCS services use BiFC property to capture and reveal protein-protein interactions in chosen human cell lines. This approach is very sensitive tool and allows the validation of weak and transient interactions at confocal or super-resolution scale.

As an example, we used this technology to characterize the interaction of developmental protein with a known cofactor and how a mutation impacts these interactions.

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I-LiPiCS

Using high-content screening or confocal microscopy, LiPiCS services studies complex disruption.

 

This approach proposes a potent alternative for large scale drug screening as LiPiCS services develops cell lines with target complex witch enables large scale molecular analysis. 

As an example, we quantified the effect of a new chemical drug on the interaction between the oncogene ERK and a newly discovered partner.

Image de Raphaël Biscaldi

S-LiPiCS

LiPiCS services uses a newly developed large scale, protein-protein interaction screening technology to identify all the protein partners of a target in customable environment and cell line. This method allows comparative analysis of protein-protein interaction network and has higher reproducibility compared to classical Yeast-two hybrid or Mass specter approaches.

 

The analysis of the networks perform by our expert inquires biological functions, pathways and protein structures involved with your protein of interest. 

As an example, we used this technology to study the effect of an anti-oncogenic peptide targeting the onco-protein C-MYC. We compared pathways and molecular functions impacted by the peptide.